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KMID : 1094720000050050345
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 5 p.345 ~ p.349
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Development of coencapsulating technology for the production of chitosanoligosaccharides
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Lee Ki-Sun
Choi Myeong-Rak
Lim Hyun-Soo
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Abstract
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To easily separate chitosanoligosaccharides by size exclusion, an coencapsulating technology of substrate and enzyme was developed. The membrane was composed of alginate and a divalent cation such as calcium. Chitosan and chitosanase were enveloped in this membrane and the product released to medium by size exclusion. The capsule was stabilized in a 2% acetic acid solution (pH 5.0) containing 0.145 M CaCO3. The leakage of substrate caused by the agitation speed was controlled by increasing alginate and CaCO3 concentrations. The lower limit of the alginate concentration and the agitation speed were 0.5% and 40 rpm, respectively. Membrane thickness and capsule diameter were 10 μm and 2.5 mm, respectively. By TLC analysis, the composition of chitosanoligosaccharides were mainly 3?6 mers. The molecular weight distribution of the released oligosaccharides ranged from 262 to 3624 Da by GPC.
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KEYWORD
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chitosanoligosaccharide, coencapsulation, size exclusion
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